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KMID : 0613819990090010084
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Journal of Life Science
1999 Volume.9 No. 1 p.84 ~ p.91
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Abstract
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Sediment samples from the waste-oil spilled sites were screened for microorganisms ablt to degrade the components crude oil, and 3 strains that could degrade were obtained. The isolated 3 strains (X1, X2 and X3) metabolized naphthalene and 2-methyl naphthalene about 80% as well as hexane and hexadecane about 60$\sim $70% as a sole carbon sources in 7 days. The degradation of the waste oil was about 60%. The addition of synthetic surfactant, Triton-X 100 or Tween 20 slightly inhibited the growth of the populations. X1 and X2 were gram negative and X3 was gram positive. X1 and X3 showed ampicillin resistancy. X1 strain having 30kb plasmid has been selected for genetic study. The plasmid was isolated and transformed into E. coli. showing the possibility of the genetically engineered degrader.
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